Bone Morphogenetic Protein 6 (BMP6) (Active) Protein

Name: Bone Morphogenetic Protein 6 (BMP6)

Synonyms: VGR, VGR1, Vgr1, D13Wsu115e, BMP6, zgc:113595, vgr, vgr1, vgr-1, MGC115308

Protein Type: Recombinant

Biological Activity: Active

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413091

Additional Information

Gene ID: 654

NCBI Acession: P22004

UniProt: P22004

Format: Lyophilized

Reconstitution: Reconstitute to a concentration of 0.1-1.0 mg/mL in sterile 20 mM acetic acid. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Endotoxin Level: <0.1 ng/μg

Description: BMPs (bone morphogenetic proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. As implied by their name, BMPs promote and regulate bone development, growth, remodeling and repair, in both prenatal development and postnatal growth of eye, heart, kidney, skin, and other tissues. Recombinant human BMP-6 produced from E. coli contains a mixture of monomer (15 kDa), dimer (30 kDa), and multimers. The dimer is an active polypeptide chain containing 2X142 amino acid residues.
Synonyms: Human BMP-6, BMP-6, BMP6, BMP 6, h-BMP-6, rh-BMP-6, recombinant human BMP-6, recombinant BMP-6, BMP, VGR, VGR1, BMP-6

Characteristics: Biological activity: 2-12 µg/mL recombinant human BMP-6 is biologically active. The ED₅₀ as determined by dose-dependent induction of alkaline phosphatase activity with C2C12 cells is in the range of 2-12 µg/mL.

Molecular Weight: monomer (15 kDa), dimer (30 kDa)

Application Details

Handling Advice: Centrifuge the vial prior to opening.

Purity: >95% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per μ g of BMP-6.

Buffer: Sterile filtered and lyophilized with no additives

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Jukes, Both, Leusink et al.: "Endochondral bone tissue engineering using embryonic stem cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 19, pp. 6840-5, 2008 (PubMed).

Crews, Adame, Patrick et al.: "Increased BMP6 levels in the brains of Alzheimer's disease patients and APP transgenic mice are accompanied by impaired neurogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 37, pp. 12252-62, 2010 (PubMed).