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Caspase 1 (CASP1) (Active) Protein

Details for Product No. ABIN413110
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Protein Name
Synonyms CG5370, DCP-1, DCP1, Dcp1, Dmel\\CG5370, ccp1, dcp-1, dcp1, l(2)01862, l(2)02132, Ice, ICE, Il1bc, IL1BC, P45, CASP1
Origin
Human
(18), (5), (5), (3), (2), (1), (1), (1)
Source
Escherichia coli (E. coli)
(23), (5), (3), (2)
Protein Type Recombinant
Biological Activity Active
Application
Western Blotting (WB), ELISA, Functional Studies (Func)
Pubmed 7 references available
Quantity 100 U
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Catalog No. ABIN413110
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Characteristics Biological activity: > 5000 U/mg One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37 °C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.
Alternative Name Caspase-1
Background Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA or Ac-YVAD-AFC (ABIN412409). The rh-Caspase has an N-term His-tag and corresponds to AA 120-404 of Caspase-1, gene accession# NP_150634.1.
Synonyms: Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, IL-1 beta-converting enzyme, p45
Molecular Weight large (20 kD) and small (10 kD) subunits
Gene ID 834
NCBI Accession NP_150634.1
Research Area Apoptosis/Necrosis
Application Notes Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity. For a complete caspase-1 assay protocol, please refer to Caspase-1 Fluorometric or Colorimetric Assay Kits.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per µL in PBS containing 15 % glycerol.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months
Product cited in: Hasegawa, Yamada, Komiyama et al.: "A novel natural compound, a cycloanthranilylproline derivative (Fuligocandin B), sensitizes leukemia cells to apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) through 15-deoxy-Delta 12, 14 prostaglandin J2 production." in: Blood, Vol. 110, Issue 5, pp. 1664-74, 2007 (PubMed).

Chen, Xia, Fang et al.: "Caspase-10-mediated heat shock protein 90 beta cleavage promotes UVB irradiation-induced cell apoptosis." in: Molecular and cellular biology, Vol. 29, Issue 13, pp. 3657-64, 2009 (PubMed).

Duncan, Gao, Huang et al.: "Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 10, pp. 6460-9, 2009 (PubMed).

Cheng, Chen, Best et al.: "The capsid proteins of Aleutian mink disease virus activate caspases and are specifically cleaved during infection." in: Journal of virology, Vol. 84, Issue 6, pp. 2687-96, 2010 (PubMed).

Murakami, Tolstykh, Bao et al.: "Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34337-47, 2010 (PubMed).

Lamkanfi, Sarkar, Vande Walle et al.: "Inflammasome-dependent release of the alarmin HMGB1 in endotoxemia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4385-92, 2010 (PubMed).

Oh, Kim, Jeong: "Distinct effects of imperatorin on allergic rhinitis: imperatorin inhibits caspase-1 activity in vivo and in vitro." in: The Journal of pharmacology and experimental therapeutics, Vol. 339, Issue 1, pp. 72-81, 2011 (PubMed).

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