You are viewing an incomplete version of our website. Please click to reload the website as full version.

Caspase 1 (CASP1) (Active) Protein

Details for Product No. ABIN413110, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Protein Name
(21), (8), (5), (4), (2), (1), (1), (1)
Escherichia coli (E. coli)
(33), (5), (2), (1), (1)
Protein Type Recombinant
Biological Activity Active
ELISA, Functional Studies (Func), Western Blotting (WB)
Pubmed 8 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 100 U
Shipping to United States ( )
Characteristics Biological activity: > 5000 U/mg One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37 °C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.
Background Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kDa) and small (10 kDa) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA or Ac-YVAD-AFC (ABIN412409). The rh-Caspase has an N-term His-tag and corresponds to AA 120-404 of Caspase-1, gene accession# NP_150634.1.
Synonyms: Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, IL-1 beta-converting enzyme, p45
Molecular Weight large (20 kD) and small (10 kD) subunits
Gene ID 834
NCBI Accession NP_150634
Research Area Apoptosis/Necrosis
Pathways Apoptosis
Application Notes Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity. For a complete caspase-1 assay protocol, please refer to Caspase-1 Fluorometric or Colorimetric Assay Kits.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per µL in PBS containing 15 % glycerol.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Caspase 1 (CASP1) (Active) Protein (ABIN413110) Caspase 1 (CASP1) (Active) protein
Product cited in: Han, Kim, Jeong: "The β-sitosterol attenuates atopic dermatitis-like skin lesions through down-regulation of TSLP." in: Experimental biology and medicine (Maywood, N.J.), Vol. 239, Issue 4, pp. 454-64, 2014 (PubMed).

Oh, Kim, Jeong: "Distinct effects of imperatorin on allergic rhinitis: imperatorin inhibits caspase-1 activity in vivo and in vitro." in: The Journal of pharmacology and experimental therapeutics, Vol. 339, Issue 1, pp. 72-81, 2011 (PubMed).

Lamkanfi, Sarkar, Vande Walle et al.: "Inflammasome-dependent release of the alarmin HMGB1 in endotoxemia." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4385-92, 2010 (PubMed).

Murakami, Tolstykh, Bao et al.: "Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977." in: The Journal of biological chemistry, Vol. 285, Issue 45, pp. 34337-47, 2010 (PubMed).

Cheng, Chen, Best et al.: "The capsid proteins of Aleutian mink disease virus activate caspases and are specifically cleaved during infection." in: Journal of virology, Vol. 84, Issue 6, pp. 2687-96, 2010 (PubMed).

Duncan, Gao, Huang et al.: "Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 10, pp. 6460-9, 2009 (PubMed).

Chen, Xia, Fang et al.: "Caspase-10-mediated heat shock protein 90 beta cleavage promotes UVB irradiation-induced cell apoptosis." in: Molecular and cellular biology, Vol. 29, Issue 13, pp. 3657-64, 2009 (PubMed).

Hasegawa, Yamada, Komiyama et al.: "A novel natural compound, a cycloanthranilylproline derivative (Fuligocandin B), sensitizes leukemia cells to apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) ..." in: Blood, Vol. 110, Issue 5, pp. 1664-74, 2007 (PubMed).

Catalog No. ABIN413110

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)