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Caspase 1 (CASP1) (Active) Protein

Details for Product No. ABIN413112
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Protein Name
Synonyms CG5370, DCP-1, DCP1, Dcp1, Dmel\\CG5370, ccp1, dcp-1, dcp1, l(2)01862, l(2)02132, Ice, ICE, Il1bc, IL1BC, P45, CASP1
Origin
Mouse (Murine)
(19), (5), (4), (3), (2), (1), (1), (1)
Source
Escherichia coli (E. coli)
(23), (5), (3), (2)
Protein Type Recombinant
Biological Activity Active
Application
Western Blotting (WB), ELISA, Functional Studies (Func)
Pubmed 2 references available
Quantity 100 U
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Catalog No. ABIN413112
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Characteristics Biological activity: >3500 U/mg One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37 °C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.
Alternative Name Caspase-1
Background Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA or Ac-YVAD-AFC (ABIN412409).
Synonyms: Active Mouse Caspase-1, Caspase-1 Enzyme, caspase-1, mouse caspase-1, mouse caspases, active caspases, caspase-1 assay positive control,
Molecular Weight large (20 kD) and small (10 kD) subunits.
Gene ID 12362
UniProt P29452
Research Area Apoptosis/Necrosis
Application Notes Active caspase-1 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity. For a complete caspase-1 assay protocol, please refer to Caspase-1 Fluorometric or Colorimetric Assay Kits.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per µL in PBS containing 15 % glycerol.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months
Product cited in: Newman, Leppla, Moayeri: "CA-074Me protection against anthrax lethal toxin." in: Infection and immunity, Vol. 77, Issue 10, pp. 4327-36, 2009 (PubMed).

Newman, Sirianni, Mawhinney et al.: "Auranofin protects against anthrax lethal toxin-induced activation of the Nlrp1b inflammasome." in: Antimicrobial agents and chemotherapy, Vol. 55, Issue 3, pp. 1028-35, 2011 (PubMed).

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