Collagen, Type I (COL1) Protein

Name: Collagen, Type I (COL1)

Protein Type: Recombinant

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413165

Additional Information

Characteristics: When adding rhCollagen (>0.03% concentration) into culture plate, rhCollagen has remarkable effect on cell proliferation, improving 40-60% in comparison to control culture.

Description: Recombinant human collagen produced from E. coli is a non-glycosylated 96 kDa polypeptide chain. It is expressed with human collagen cDNA transcribed reversely from mRNA and purified by proprietary chromatographic techniques. Recombinant human Collage remains the function of collagen and unlike collagen extracted from animal tissues, rhCollagen is free from virus, water soluble, and with less immunogenecity for in vivo studies.

Molecular Weight: 96 kDa

Application Details

Purity: > 95% as determined by SDS-PAGE and HPLC. Endotoxin level is less than 0.1 ng/mg of collagen PHYSICAL

Storage: The lyophilized product is best stored at –20 °C. Reconstituted solution can be stored at 4 °C for 2-5 days. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA) and keep desiccated below –20 °C. Avoid freeze-thaw cycles. Reconstitution: Centrifuge the vial prior to opening. Reconstitute the lyophilized rhCollagen in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted to other aqueous solutions and stored aliquoted at –20 °C for future use.

Restrictions: For Research Use only