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Epidermal Growth Factor (EGF) (Active) Protein

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Protein Name
Origin
Human
(82), (38), (27), (10), (4), (4), (1), (1)
Source
Escherichia coli (E. coli)
(117), (8), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1)
Protein Type Recombinant
Biological Activity Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Pubmed 2 references available
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Quantity 5 mg
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Specificity The ED50 as determined by the dose-dependent stimulation of the proliferation of thymidine uptake by BALB/c 3T3 cells is < 0.1 ng/mL, corresponding to a specific activity of > 1 x 10^7 U/mg, Results: < 0.1 ng/mL, Binding Capacity:
Characteristics Biological Activity: The ED₅₀ of human EGF is determined by the dose-dependent stimulation of BALB/c 3T3 cells is 5.92-10.06 ng/mL.
Purity ≥98 %
Endotoxin Level <0.1 ng/μg
Background EGF (Epidermal Growth Factor) is polypeptide growth factor that stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human EGF is a 6.2 kDa protein containing 53 amino acid residues
Synonyms: human EGF, EGF, h-EGF, rh-EGF, recombinant human EGF, recombinant EGF, Urogastrone, URG, EGF, epidermal growth factor
Molecular Weight 6.2 kDa
UniProt Q6QBS2
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Comment

physical form description: EGF protein is lyophilized with no additives

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute the human EGF in H₂O to a concentration of 0.1-1 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Supplier Images
Image no. 1 for Epidermal Growth Factor (EGF) (Active) Protein (ABIN413182) Epidermal Growth Factor (EGF) (Active) protein
Product cited in: Wang, Qian, Li et al.: "Evaluation of emulsion electrospun polycaprolactone/hyaluronan/epidermal growth factor nanofibrous scaffolds for wound healing." in: Journal of biomaterials applications, 2015 (PubMed).

An, Gip, Kim et al.: "Extensive determination of glycan heterogeneity reveals an unusual abundance of high mannose glycans in enriched plasma membranes of human embryonic stem cells." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 4, pp. M111.010660, 2012 (PubMed).

Catalog No. ABIN413182
$1,072.50
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