You are viewing an incomplete version of our website. Please click to reload the website as full version.

Follistatin (FST) (Active) Protein

Details for Product No. ABIN413280, Supplier: Login to see
Protein Name
Origin
Human
34
5
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
19
9
4
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Specificity Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Activin A. Results: 0.1-0.4 µg/mL.
Characteristics Biological Activity: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED₅₀ is 0.1-0.4 μg/mL in the presence of 7.5 ng/mL Activin A.
Purity ≥95 %
Sterility Sterile filtered
Endotoxin Level <0.1 ng/μg
Background Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-beta ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-beta1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.
Synonyms: Human Follistatin, Follistatin, h-Follistatin, rh-Follistatin, recombinant human Follistatin, recombinant Follistatin, Follistatin, FST, FS, Activin-binding protein
Molecular Weight 31.5 kDa
Gene ID 10468
UniProt P19883
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Hormones
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in H₂O to a concentration of 0.1-1.0 μg/μl. The solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Buffer Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Product cited in: Li, Shimono, Norioka et al.: "Activin A binds to perlecan through its pro-region that has heparin/heparan sulfate binding activity." in: The Journal of biological chemistry, Vol. 285, Issue 47, pp. 36645-55, 2010 (PubMed).

Umezu, Yamanouchi, Iida et al.: "Follistatin-like-1, a diffusible mesenchymal factor determines the fate of epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 10, pp. 4601-6, 2010 (PubMed).

Rose, Mattis, Rindt et al.: "Delivery of recombinant follistatin lessens disease severity in a mouse model of spinal muscular atrophy." in: Human molecular genetics, Vol. 18, Issue 6, pp. 997-1005, 2009 (PubMed).