Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) Protein

Name: Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2)

Synonyms: GMCSF, MGC131935, MGC138897, Csfgm, Gm-CSf, MGI-IGM, MGC151255, MGC151257, Gmcsf, Gm-csf, CSF2, GM-CSF

Protein Type: Recombinant

Biological Activity: Active

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413328

Additional Information

Gene ID: 1437

NCBI Acession: P04141

UniProt: P04141

Format: Lyophilized

Reconstitution: Reconstitute in H₂O to a concentration of 0.1-1.0 µg/µL. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Endotoxin Level: <0.1 ng/μg

Description: Human GM-CSF (Granulocyte Macrophage Colony stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein containing 128 amino acid residues.
Synonyms: human GM-CSF, GM-CSF,GMCSF, GM CSF, h-GM-CSF, rh-GM-CSF, recombinant human GM-CSF, recombinant GM-CSF, Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-alpha

Characteristics: Biological activity: <0.1 ng/mL, The ED₅₀ determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.1 ng/mL, corresponding to a specific activity of >1 x 10⁷ units/mg.

Molecular Weight: 14.6 kDa

Application Details

Handling Advice: Centrifuge the vial prior to opening.

Purity: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per μ g GM-CSF.

Buffer: Lyophilized from 10 mM Sodium Citrate, pH 3.5

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Santegoets, Bontkes, Stam et al.: "Inducing antitumor T cell immunity: comparative functional analysis of interstitial versus Langerhans dendritic cells in a human cell line model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4540-9, 2008 (PubMed).

van de Ven, Scheffer, Reurs et al.: "A role for multidrug resistance protein 4 (MRP4; ABCC4) in human dendritic cell migration." in: Blood, Vol. 112, Issue 6, pp. 2353-9, 2008 (PubMed).

Kinzfogl, Hangoc, Broxmeyer: "Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells." in: Blood, Vol. 118, Issue 3, pp. 565-75, 2011 (PubMed).