GM-CSF (Active) Protein

Details for Product No. ABIN413327, Supplier: Log in to see
Protein Name
  • GM-CSF
  • granulocyte-macrophage colony-stimulating-factor
  • granulocyte-macrophage colony-stimulating factor
  • GM-CSF
Origin
Human
45
21
11
2
1
1
Source
Escherichia coli (E. coli)
63
5
4
4
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Purity ≥98 % by SDS-PAGE
≥98 % by HPLC
Endotoxin Level <0.1 ng/μg
ProductDetails: Biological Activity Comment The ED₅₀ determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <0.1 ng/ml, corresponding to a specific activity of >1 x 10⁷ units/mg.
Background Human GM-CSF (Granulocyte Macrophage Colony stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein containing 128 amino acid residues.

Alternative Names: Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-α, Colony-stimulating factor, Molgramostin, Sargramostim
Molecular Weight 14.6 kDa
Gene ID 1437
UniProt P04141
Research Area Immunology, Innate Immunity, Cytokines
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in H₂O to a concentration of 0.1-1.0 μg/μl. This solution can then be diluted into other aqueous buffers or stored at 4 °C for 1 week or -20 °C for future use.
Buffer Lyophilized from 10 mM Sodium Citrate, pH 3.5
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Product cited in: Kinzfogl, Hangoc, Broxmeyer: "Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells." in: Blood, Vol. 118, Issue 3, pp. 565-75, 2011 (PubMed).

Santegoets, Bontkes, Stam, Bhoelan, Ruizendaal, van den Eertwegh, Hooijberg, Scheper, de Gruijl: "Inducing antitumor T cell immunity: comparative functional analysis of interstitial versus Langerhans dendritic cells in a human cell line model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 7, pp. 4540-9, 2008 (PubMed).

van de Ven, Scheffer, Reurs, Lindenberg, Oerlemans, Jansen, Gillet, Glasgow, Pereboev, Curiel, Scheper, de Gruijl: "A role for multidrug resistance protein 4 (MRP4; ABCC4) in human dendritic cell migration." in: Blood, Vol. 112, Issue 6, pp. 2353-9, 2008 (PubMed).