GM-CSF Protein

Name: GM-CSF

Synonyms: GMCSF, GM-CSF

Protein Type: Recombinant

Source: Escherichia Coli (E. coli)

Origin (Gene): Rat (Rattus)

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413333

Additional Information

Characteristics: The ED 50 determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is <0.01 ng/ml, corresponding to a specific activity of >1 x 10 8 units/mg.

Description: Rat GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages and eosinophils. Recombinant rat GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acid residues.

Molecular Weight: 14.5 kDa

Application Details

Purity: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per μ g GM-CSF.

Buffer: Sterile filtered and lyophilized from 10 mM Tris, pH 8.5.

Storage: The lyophilized rat GM-CSF is best-stored desiccated below 0 °C. Reconstituted GM-CSF should be stored in working aliquots at -20 °C. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4 °C for 1 week or – 20 °C for future use.

Restrictions: For Research Use only