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Interleukin 18 (IL18) (Active) Protein

Details for Product No. ABIN413466, Supplier: Log in to see
Protein Name
  • Igif
  • Il-18
  • IL-18
  • IGIF
  • ChIL-18
  • IL-1g
  • IL1F4
  • IL18
  • interleukin 18
  • interleukin 18 (interferon-gamma-inducing factor)
  • interleukin-18
  • Il18
  • IL18
  • Il-18
Origin
Human
32
14
12
6
2
2
2
2
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
51
9
2
2
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Purity ≥98 % by SDS-PAGE
≥98 % by HPLC
Sterility Sterile filtered
Endotoxin Level <0.1 ng/μg
ProductDetails: Biological Activity Comment The ED₅₀ as determined by dose-dependent stimulation of the IFNg by human PBMC co-stimulated with rh-IL-12 was found to be 5 ng/ml.
Background Interleukin-18 (IL-18), an 18 kDa cytokine, is a single, non-glycosylated polypeptide chain containing 157 amino acids. IL-18 was previously known as interferon-g inducing factor (IGIF). This pleiotropic cytokine is produced by monocyte/macrophage cells. Like IL-12, IL-18 plays an important role in cell-mediated immune responses.

Alternative Names: Interferon-γ-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-γ-inducing factor, Interleukin-1 γ, IL-1 γ, Iboctadekin.
Molecular Weight 18.3 kDa
Gene ID 3606
UniProt Q14116
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in ice-cold H₂O to a concentration of 0.5-1 μg/μl. The solution can be stored at 4 °C for 1 week. For storage, prepare appropriate aliquots and freeze them at -80 °C for future use.
Buffer Sterile filtered and lyophilized from PBS
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Product cited in: Moriwaki, Bertin, Gough, Chan: "A RIPK3-caspase 8 complex mediates atypical pro-IL-1β processing." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 4, pp. 1938-44, 2015 (PubMed).

Uzan, Poglio, Gerby, Wu, Gross, Armstrong, Calvo, Cahu, Deswarte, Dumont, Passaro, Besnard-Guérin, Leblanc, Baruchel, Landman-Parker, Ballerini, Baud, Ghysdael, Baleydier, Porteu, Pflumio: "Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression." in: EMBO molecular medicine, Vol. 6, Issue 6, pp. 821-34, 2014 (PubMed).

Kahlenberg, Carmona-Rivera, Smith, Kaplan: "Neutrophil extracellular trap-associated protein activation of the NLRP3 inflammasome is enhanced in lupus macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1217-26, 2013 (PubMed).

Kahlenberg, Thacker, Berthier, Cohen, Kretzler, Kaplan: "Inflammasome activation of IL-18 results in endothelial progenitor cell dysfunction in systemic lupus erythematosus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 6143-56, 2011 (PubMed).