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Interleukin 18 (IL18) (Active) Protein

Details for Product No. ABIN413466, Supplier: Login to see
Protein Name
Origin
Human
25
12
10
2
2
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
35
10
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Characteristics 18.3 kDa
Biological Activity: The ED₅₀ as determined by dose-dependent stimulation of the IFNg by human PBMC co-stimulated with rh-IL-12 was found to be 5 ng/mL.
Purity ≥98 %
Sterility Sterile filtered
Endotoxin Level <0.1 ng/μg
Background Interleukin-18 (IL-18), an 18 kDa cytokine, is a single, non-glycosylated polypeptide chain containing 157 amino acids. IL-18 was previously known as interferon-g inducing factor (IGIF). This pleiotropic cytokine is produced by monocyte/macrophage cells. Like IL-12, IL-18 plays an important role in cell-mediated immune responses.
Synonyms: Human IL-18, IL-18, IL18, IL 18, h-IL-18, rh-IL-18, recombinant human IL-18, recombinant IL-18, IL, interluekin, Interferon-gamma-inducing factor, IGIF, IL-1g, IL-18, IL1F4, MGC12320, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma, Iboctadekin.
Molecular Weight 18.3 kDa
Gene ID 3606
UniProt Q14116
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in ice-cold H₂O to a concentration of 0.5-1 μg/μl. The solution can be stored at 4 °C for 1 week. For storage, prepare appropriate aliquots and freeze them at -80 °C for future use.
Buffer Sterile filtered and lyophilized from PBS
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Product cited in: Moriwaki, Bertin, Gough et al.: "A RIPK3-caspase 8 complex mediates atypical pro-IL-1β processing." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 4, pp. 1938-44, 2015 (PubMed).

Uzan, Poglio, Gerby et al.: "Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression." in: EMBO molecular medicine, Vol. 6, Issue 6, pp. 821-34, 2014 (PubMed).

Kahlenberg, Carmona-Rivera, Smith et al.: "Neutrophil extracellular trap-associated protein activation of the NLRP3 inflammasome is enhanced in lupus macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 3, pp. 1217-26, 2013 (PubMed).

Kahlenberg, Thacker, Berthier et al.: "Inflammasome activation of IL-18 results in endothelial progenitor cell dysfunction in systemic lupus erythematosus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 6143-56, 2011 (PubMed).