Interleukin 1, beta (IL1B) (Active) Protein

Name: Interleukin 1, beta (IL1B)

Synonyms: IL-1, IL1F2, IL1-BETA, IL1B, IL-1BETA, MGC111873, zgc:111873, IL-1B

Protein Type: Recombinant

Biological Activity: Active

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413478

Additional Information

Gene ID: 3553

NCBI Acession: P01584

UniProt: P01584

Format: Lyophilized

Reconstitution: We recommend a quick spin followed by reconstitution in H₂O to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or stored at –20°C for future use.

Endotoxin Level: <0.1 ng/μg

Description: IL-1beta is a potent immuno-modulator that mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Recombinant human IL-1beta is a 17.0 kDa protein containing 153 amino acid residues.
Synonyms: Human IL-1beta, IL-1beta, IL1beta, IL 1beta, h- IL-1beta, rh-IL-1beta, recombinant human IL-1beta, recombinant IL-1beta, IL, interluekin, Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Characteristics: Biological activity: <0.1 ng/mL The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by murine C3H/HeJ thymocytes is <0.1 ng/mL, corresponding to a specific activity of >1 x 10⁷ units/mg.

Molecular Weight: 17.0 kDa

Application Details

Handling Advice: Centrifuge the vial prior to opening.

Purity: >98% by SDS-PAGE and HPLC analysis Endotoxin level is <0.1 ng per μg of IL-1beta.

Buffer: Lyophilized with no additives

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Kahlenberg, Thacker, Berthier et al.: "Inflammasome activation of IL-18 results in endothelial progenitor cell dysfunction in systemic lupus erythematosus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 6143-56, 2011 (PubMed).