PDGF-AB (PDGF-AB) Protein

Name: PDGF-AB (PDGF-AB)

Protein Type: Recombinant

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413703

Additional Information

Characteristics: The ED 50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is < 1 ng/ml, corresponding to a specific activity of >1 x 10 6 units/mg.

Description: Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain.

Molecular Weight: 25.5 kDa

Application Details

Purity: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per μ g.

Buffer: Lyophilized with no additives

Storage: The lyophilized protein is best-stored desiccated below 0 °C. Reconstituted human PDGF-AB should be stored in working aliquots at -20 °C. Reconstitution: Centrifuge the vial prior to opening. Reconstitute in 10 mM acetic acid to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or –20 °C for future use.

Restrictions: For Research Use only