Tumor Necrosis Factor (TNF) (Active) Protein

Details for Product No. ABIN413823, Supplier: Log in to see
Protein Name
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Origin
Mouse (Murine)
115
49
29
9
6
5
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
155
26
16
6
4
3
3
2
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Options
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Purity ≥98 % by SDS-PAGE
≥98 % by HPLC
Endotoxin Level <0.1 ng/μg
ProductDetails: Biological Activity Comment The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 10⁷ units/mg.
Background Tumor Necrosis Factor-α TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Murine TNF-α is a 17.5 kDa protein containing 156 amino acid residues.

Alternative Names: Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Molecular Weight 17.5 kDa
Gene ID 21926
UniProt P06804
Research Area Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in H₂O to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or -20 °C for future use.
Buffer Steriled and lyophilized from 5 mM Tris, pH 8.0.
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Tumor Necrosis Factor (TNF) (Active) protein (ABIN413823) Tumor Necrosis Factor (TNF) (Active) protein
Product cited in: Srivastava, Qin, Wedhas, Arnush, Linkhart, Chadwick, Kumar: "Tumor necrosis factor-alpha augments matrix metalloproteinase-9 production in skeletal muscle cells through the activation of transforming growth factor-beta-activated kinase 1 (TAK1)-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 35113-24, 2007 (PubMed).

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