Thyroid Peroxidase (TPO) (Active) Protein

Name: Thyroid Peroxidase (TPO)

Synonyms: MSA, TPX, TDH2A, TPO, msa, tpx, xtpo, LOC100218312

Protein Type: Recombinant

Biological Activity: Active

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413832

Additional Information

Gene ID: 7173

NCBI Acession: P07202

UniProt: P07202

Format: Lyophilized

Reconstitution: Reconstitution in purified H₂O to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Endotoxin Level: <0.1 ng/μg

Description: TPO (Thrombopoietin, also known as Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant human TPO is a fully biologically active 174 amino acid polypeptide, which contains the erythropoietin-like domain of the full length TPO protein.
Synonyms: Human TPO, TPO, h-TPO, rh-TPO, recombinant human TPO, recombinant TPO, TPO, Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Characteristics: Biological activity: <1 ng/mL The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found <1 ng/mL, corresponding to a specific activity of >1 x 10⁶ IU/mg.

Application Details

Handling Advice: Centrifuge the vial prior to opening.

Purity: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per μ g of TPO.

Buffer: Lyophilized with no additives

Storage: -20°C

Expiry Date: 12 months

Restrictions: For Research Use only

Publications

Product: Kinzfogl, Hangoc, Broxmeyer: "Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells." in: Blood, Vol. 118, Issue 3, pp. 565-75, 2011 (PubMed).