Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Active) Protein

Details for Product No. ABIN413893, Supplier: Log in to see
Protein Name
  • Xint-1
  • Xwnt1
  • int-1
  • int1
  • wnt-1
  • wnt1-a
  • Int-1
  • Wnt-1
  • sw
  • swaying
  • BMND16
  • INT1
  • OI15
  • Int1
  • WNT-1
  • sb:eu647
  • zgc:194464
  • zgc:194478
  • WNT1
  • wingless-type MMTV integration site family, member 1
  • wingless-related MMTV integration site 1
  • WNT1
  • wnt1
  • Wnt1
  • WNT-1
Origin
Human
8
Source
Escherichia coli (E. coli)
6
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Purity ≥98 % by SDS-PAGE
≥98 % by HPLC
Sterility Sterile filtered
Endotoxin Level <0.1 ng/μg
ProductDetails: Biological Activity Comment The ED₅₀ was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED₅₀ for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Background Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intraCellular signaling protein, β-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.

Alternative Names: INT-1, Proto-oncogene Int-1 homolog
Molecular Weight 38.4 kDa
Gene ID 7471
UniProt P04628
Pathways WNT Signaling, Dopaminergic Neurogenesis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in H₂O to a concentration of 0.1-1.0 μg/μl. The solution can then be diluted into other aqueous buffers
Buffer Sterile filtered and lyophilized with no additives
Preservative Without preservative
Handling Advice Centrifuge the vial prior to opening.
Storage -20 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Active) Protein (ABIN413893) Wingless-Type MMTV Integration Site Family, Member 1 (WNT1) (Active) protein
Product cited in: Reuter, Martin, Beckert, Bros, Montermann, Belz, Heinz, Ohngemach, Sahin, Stassen, Buhl, Eshkind, Taube: "The Wnt/β-catenin pathway attenuates experimental allergic airway disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 2, pp. 485-95, 2014 (PubMed).

Tawk, Makoukji, Belle, Fonte, Trousson, Hawkins, Li, Ghandour, Schumacher, Massaad: "Wnt/beta-catenin signaling is an essential and direct driver of myelin gene expression and myelinogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 10, pp. 3729-42, 2011 (PubMed).