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Glutathione S Transferase (GST) (Active) Protein

Details for Product No. ABIN413895, Supplier: Log in to see
Protein Name
  • GST
  • GST 13-13
  • GST13
  • GST13-13
  • GSTK1-1
  • hGSTK1
  • GSTS
  • GSTS1-1
  • PGD2
  • PGDS
  • H-PGDS
  • Ptgds2
  • 1500002K10Rik
  • Gst
  • glutathione S-transferase kappa 1
  • hematopoietic prostaglandin D synthase
  • microsomal glutathione S-transferase 1
  • GSTK1
  • HPGDS
  • Hpgds
  • Mgst1
Origin
Rat (Rattus)
10
4
4
2
2
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
18
10
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Purpose Pure Active GST protein, for positive control in GST activity assay or for screening GST inhibitors.
Purification Purified from equine liver
ProductDetails: Biological Activity Comment Biological Activity: 300,000 units/mg
Unit Definition One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Background As a positive control in GST activity assay or for screening GST inhibitors. Refer to the GST Fluorometric and Colorimetric Assay Kits for detailed assay procedures .
Alternate Names: GST, Glutathione S-Transferase, Glutathione Transferase
CAS-No 50812-37-8
Research Area Metabolism, Immunology
Application Notes As a positive control in GST activity assay or for screening GST inhibitors. Refer to the GST Fluorometric and Colorimetric Assay Kits for detailed assay procedures.
Optimal working dilution should be determined by the investigator.
Comment

physical form description: Lyophilized powder

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per μl in PBS containing 15 % glycerol.
Buffer 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1 % Chaps, 10 mM EDTA, 5 % Glycerol, and 10 mM DTT.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -20°C
Expiry Date 12 months
Product cited in: Ji, Tulin: "Poly(ADP-ribosyl)ation of heterogeneous nuclear ribonucleoproteins modulates splicing." in: Nucleic acids research, Vol. 37, Issue 11, pp. 3501-13, 2009 (PubMed).

Candelario-Jalil, Taheri, Yang, Sood, Grossetete, Estrada, Fiebich, Rosenberg: "Cyclooxygenase inhibition limits blood-brain barrier disruption following intracerebral injection of tumor necrosis factor-alpha in the rat." in: The Journal of pharmacology and experimental therapeutics, Vol. 323, Issue 2, pp. 488-98, 2007 (PubMed).