Glutathione S Transferase (GST) (Active) Protein

Details for Product No. ABIN413895, Supplier: Log in to see
Protein Name
  • GST
  • GST 13-13
  • GST13
  • GST13-13
  • GSTK1-1
  • hGSTK1
  • GSTS
  • GSTS1-1
  • PGD2
  • PGDS
  • H-PGDS
  • Ptgds2
  • 1500002K10Rik
  • Gst
  • glutathione S-transferase kappa 1
  • hematopoietic prostaglandin D synthase
  • microsomal glutathione S-transferase 1
  • GSTK1
  • HPGDS
  • Hpgds
  • Mgst1
Origin
Rat (Rattus)
11
4
4
2
1
1
1
1
1
1
1
1
1
1
Source
Escherichia coli (E. coli)
18
10
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
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Purpose Pure Active GST protein, for positive control in GST activity assay or for screening GST inhibitors.
Characteristics Molecule Information: large (17 kD) and small (11 kD) subunits
Purification Purified from equine liver
ProductDetails: Biological Activity Comment Specification / Test Method: 300,000 units/mg
Unit Definition One unit of the recombinant caspase-3 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
Background Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme. The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).

Alternative Names: Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, IRP, LICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1
CAS-No 50812-37-8
Research Area Apoptosis/Necrosis, Autophagy
Application Notes As a positive control in GST activity assay or for screening GST inhibitors. Refer to the GST Fluorometric and Colorimetric Assay Kits for detailed assay procedures.
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute to 1 unit per μl in PBS containing 15 % glycerol.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -20°C
Expiry Date 12 months
Product cited in: Ji, Tulin: "Poly(ADP-ribosyl)ation of heterogeneous nuclear ribonucleoproteins modulates splicing." in: Nucleic acids research, Vol. 37, Issue 11, pp. 3501-13, 2009 (PubMed).

Candelario-Jalil, Taheri, Yang, Sood, Grossetete, Estrada, Fiebich, Rosenberg: "Cyclooxygenase inhibition limits blood-brain barrier disruption following intracerebral injection of tumor necrosis factor-alpha in the rat." in: The Journal of pharmacology and experimental therapeutics, Vol. 323, Issue 2, pp. 488-98, 2007 (PubMed).