B3GNT1 Protein (AA 43-415) (His tag)

Catalog No. ABIN4370820

Product Details

Target: B3GNT1 (UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1))

Protein Type: Recombinant

Protein Characteristics: AA 43-415

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This B3GNT1 protein is labelled with His tag.

Purpose: Recombinant Human B3GNT1 (C-6His)

Sequence: DQYFEFFPPS PRSVDQVKAQ LRTALASGGV LDASGDYRVY RGLLKTTMDP NDVILATHAS VDNLLHLSGL LERWEGPLSV SVFAATKEEA QLATVLAYAL SSHCPDMRAR VAMHLVCPSR YEAAVPDPRE PGEFALLRSC QEVFDKLARV AQPGINYALG TNVSYPNNLL RNLAREGANY ALVIDVDMVP SEGLWRGLRE MLDQSNQWGG TALVVPAFEI RRARRMPMNK NELVQLYQVG EVRPFYYGLC TPCQAPTNYS RWVNLPEESL LRPAYVVPWQ DPWEPFYVAG GKVPTFDERF RQYGFNRISQ ACELHVAGFD FEVLNEGFLV HKGFKEALKF HPQKEAENQH NKILYRQFKQ ELKAKYPNSP RRCVDHHHHH H

Characteristics: Recombinant Human N-Acetyllactosaminide beta-1,3-N-Acetylglucosaminyltransferase/B3GNT1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp43-Cys415) of Human B3GNT1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: B3GNT1 (UDP-GlcNAc:betaGal beta-1,3-N-Acetylglucosaminyltransferase 1 (B3GNT1))

Alternative Name: beta-1-3-N-Acetylglucosaminyltransferase-1 (B3GNT1 Products)

Synonyms: B3GN-T2 Protein, B3GNT Protein, B3GNT-2 Protein, B3GNT1 Protein, BETA3GNT Protein, BGNT2 Protein, BGnT-2 Protein, B3GNT2 Protein, B3GN-T1 Protein, B3GNT6 Protein, BETA3GNTI Protein, MDDGA13 Protein, iGAT Protein, iGNT Protein, 1500032M01Rik Protein, B3gnt6 Protein, BETA3GNT1 Protein, B3gnt1 Protein, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Protein, beta-1,4-glucuronyltransferase 1 Protein, B3GNT2 Protein, B4GAT1 Protein, B4gat1 Protein

Sub Type: Fusionprotein

Background: N-Acetyllactosaminide beta-1,3-N-Acetylglucosaminyltransferase (B3GNT1) is a member of the beta-1,3-N-Acetylglucosaminyltransferase family. B3GNT1 is a single-pass type II membrane protein and widely expressed in many tissues. B3GNT1 can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans. B3GNT1 is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. It can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.
Alternative Names: N-Acetyllactosaminide Beta-1,3-N-Acetylglucosaminyltransferase, I-Beta-1,3-N-Acetylglucosaminyltransferase, iGnT, Poly-N-Acetyllactosamine Extension Enzyme, UDP-GlcNAc:BetaGal Beta-1,3-N-Acetylglucosaminyltransferase 1, B3GNT1, B3GNT6

Molecular Weight: 43.4 kDa

UniProt: O43505

Pathways: Glycosaminoglycan Metabolic Process