Albumin (ALB) Protein

Name: Albumin (ALB)

Synonyms: ALB, MGC179277, alb, pro0883, pro0903, pro1341, MGC88928, CSA, DKFZp459F2310, PRO0883, PRO0903, PRO1341, DKFZp779N1935, Alb1, Alb-1, Albza, LOC100136344

Origin (Gene): Sheep (Ovine)

Application: Immunodiffusion (ID), Cell-binding assay (CBA), Haemagglutination (Haema), Dot Blot (Dot), Western Blotting (WB), ELISA, Immunoelectrophoresis (IEP)

Catalog no.: ABIN458790

Additional Information

Characteristics: Chromatographically purified sheep albumin

Source (Details): Source: Sheep serum

Description: Albumin is a stable small polypeptide with a strong antigenicity. It has a molecular weight of 69,000 and an isoelectric point of 5.7. The protein is isolated from serum by sequential precipitation and further purified by ion exchange chromatography and affinity chromatography. The identity is confirmed and tested by immunoelectrophoresis and radial immuno-diffusion using antisera to total serum proteins and albumin.

Application Details

Application Notes: Purified sheep albumin may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.

Purity: Not less than 98% as determined by agar-gel electrophoresis. Tested in a concentration of 40 mg/ml at the level of sensitivity of the applied immunoprecipitating techniques, this product contains a trace of an alpha-1 protein.

Buffer: Lyophilized, stable white powder. Preservative: No preservative added.

Storage: Lyophilized at +4° C reconstituted at or below -20° C reconstituted at +4° C at least 10 years 3-5 years 7 days. This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. After opening the vial, the lyophilized content is reconstituted by adding 2 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.

Restrictions: For Research Use only