SCLY Protein (AA 1-445) (His tag)
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- Target See all SCLY Proteins
- SCLY (Selenocysteine Lyase (SCLY))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-445
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SCLY protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human SCLY Protein is produced by our E. coli expression system and the target gene encoding Met1-Ala445 is expressed with a 6His tag at the N-terminus.
- Sequence
- MGSSHHHHHH SSGLVPRGSH MEAAVAPGRD APAPAASQPS GCGKHNSPER KVYMDYNATT PLEPEVIQAM TKAMWEAWGN PSSPYSAGRK AKDIINAARE SLAKMIGGKP QDIIFTSGGT ESNNLVIHSV VKHFHANQTS KGHTGGHHSP VKGAKPHFIT SSVEHDSIRL PLEHLVEEQV AAVTFVPVSK VSGQTEVDDI LAAVRPTTRL VTIMLANNET GIVMPVPEIS QRIKALNQER VAAGLPPILV HTDAAQALGK QRVDVEDLGV DFLTIVGHKF YGPRIGALYI RGLGEFTPLY PMLFGGGQER NFRPGTENTP MIAGLGKAAE LVTQNCEAYE AHMRDVRDYL EERLEAEFGQ KRIHLNSQFP GTQRLPNTCN FSIRGPRLQG HVVLAQCRVL MASVGAACHS DHGDQPSPVL LSYGVPFDVA RNALRLSVGR STTRAEVDLV VQDLKQAVAQ LEDQA
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product SCLY Protein
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Selenocysteine Lyase (SCLY) (AA 1-445) protein (His tag)
SCLY Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Selenocysteine Lyase (SCLY) (AA 1-445) protein (GST tag)SCLY Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Selenocysteine Lyase (SCLY) (AA 346-444) protein (GST tag)SCLY Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Selenocysteine Lyase (SCLY) (AA 1-445) protein (Strep Tag)Crystallography grade SCLY Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Selenocysteine Lyase (SCLY) (AA 1-432) protein (Strep Tag)Crystallography grade SCLY Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Selenocysteine Lyase (SCLY) protein (Myc-DYKDDDDK Tag)SCLY Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- SCLY (Selenocysteine Lyase (SCLY))
- Alternative Name
- SCLY (SCLY Products)
- Synonyms
- SCL Protein, hSCL Protein, 9830169H08 Protein, A930015N15Rik Protein, Scly1 Protein, Scly2 Protein, zgc:171514 Protein, selenocysteine lyase Protein, selenocysteine lyase L homeolog Protein, Selenocysteine lyase Protein, SCLY Protein, Scly Protein, scly.L Protein, csdB Protein, EUBELI_20129 Protein, Deima_1599 Protein, scly Protein
- Gene ID
- 51540
- UniProt
- Q96I15
- Pathways
- Stem Cell Maintenance
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