Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Protein Type
- Recombinant
- Protein Characteristics
- AA 26-421
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human ACADM Protein is produced by our E. coli expression system and the target gene encoding Lys26-Asn421 is expressed with a 6His tag at the N-terminus.
- Sequence
- MGSSHHHHHH SSGLVPRGSH MKANRQREPG LGFSFEFTEQ QKEFQATARK FAREEIIPVA AEYDKTGEYP VPLIRRAWEL GLMNTHIPEN CGGLGLGTFD ACLISEELAY GCTGVQTAIE GNSLGQMPII IAGNDQQKKK YLGRMTEEPL MCAYCVTEPG AGSDVAGIKT KAEKKGDEYI INGQKMWITN GGKANWYFLL ARSDPDPKAP ANKAFTGFIV EADTPGIQIG RKELNMGQRC SDTRGIVFED VKVPKENVLI GDGAGFKVAM GAFDKTRPVV AAGAVGLAQR ALDEATKYAL ERKTFGKLLV EHQAISFMLA EMAMKVELAR MSYQRAAWEV DSGRRNTYYA SIAKAFAGDI ANQLATDAVQ ILGGNGFNTE YPVEKLMRDA KIYQIYEGTS QIQRLIVARE HIDKYKN
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 1-100) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 1-421) protein (GST tag)Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Crystallography grade Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (AA 26-421) protein (His tag)Crystallography grade Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM Tris, 0.1M NaCl, 20 % Glycerol, pH 8.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Alternative Name
- ACADM
- Synonyms
- ACAD1 Protein, MCAD Protein, MCADH Protein, AU018656 Protein, acyl-CoA dehydrogenase medium chain Protein, acyl-Coenzyme A dehydrogenase, medium chain Protein, acyl-CoA dehydrogenase, C-4 to C-12 straight chain Protein, ACADM Protein, Acadm Protein
- Gene ID
- 34
- UniProt
- P11310
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