USP14 Protein (AA 91-494) (His tag)

Catalog No. ABIN7313956

Product Details

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Protein Type: Recombinant

Protein Characteristics: AA 91-494

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This USP14 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human USP14 Protein is produced by our E. coli expression system and the target gene encoding Asp91-Gln494 is expressed with a 6His tag at the N-terminus.

Sequence: MGSSHHHHHH SSGLVPRGSH MDMTEEQLAS AMELPCGLTN LGNTCYMNAT VQCIRSVPEL KDALKRYAGA LRASGEMASA QYITAALRDL FDSMDKTSSS IPPIILLQFL HMAFPQFAEK GEQGQYLQQD ANECWIQMMR VLQQKLEAIE DDSVKETDSS SASAATPSKK KSLIDQFFGV EFETTMKCTE SEEEEVTKGK ENQLQLSCFI NQEVKYLFTG LKLRLQEEIT KQSPTLQRNA LYIKSSKISR LPAYLTIQMV RFFYKEKESV NAKVLKDVKF PLMLDMYELC TPELQEKMVS FRSKFKDLED KKVNQQPNTS DKKSSPQKEV KYEPFSFADD IGSNNCGYYD LQAVLTHQGR SSSSGHYVSW VKRKQDEWIK FDDDKVSIVT PEDILRLSGG GDWHIAYVLL YGPRRVEIME EESEQ

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 20 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Alternative Name: USP14 (USP14 Products)

Synonyms: TGT Protein, DDBDRAFT_0202821 Protein, DDBDRAFT_0237695 Protein, DDB_0202821 Protein, DDB_0237695 Protein, 2610005K12Rik Protein, 2610037B11Rik Protein, AW107924 Protein, C78769 Protein, ax Protein, zgc:55949 Protein, wu:fk63d09 Protein, MGC81945 Protein, tgt Protein, MGC69343 Protein, USP14 Protein, ubiquitin specific peptidase 14 Protein, peptidase C19 family protein Protein, ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) Protein, ubiquitin specific protease 14 Protein, ubiquitin specific peptidase 14 S homeolog Protein, universal stress protein Protein, Ubiquitin carboxyl-terminal hydrolase 14 Protein, USP14 Protein, usp14 Protein, Usp14 Protein, Bm1_28765 Protein, usp14.S Protein, RR_RS06480 Protein, usp-14 Protein

Gene ID: 9097

UniProt: P54578