ATG3 Protein (AA 1-287) (T7 tag,His tag)
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- Target See all ATG3 Proteins
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-287
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ATG3 protein is labelled with T7 tag,His tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human ATG3 Protein is produced by our E. coli expression system and the target gene encoding Met1-His287 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
- Sequence
- MASMTGGQQM GRGSMQNVIN TVKGKALEVA EYLTPVLKES KFKETGVITP EEFVAAGDHL VHHCPTWQWA TGEELKVKAY LPTGKQFLVT KNVPCYKRCK QMEYSDELEA IIEEDDGDGG WVDTYHNTGI TGITEAVKEI TLENKDNIRL QDCSALCEEE EDEDEGEAAD MEEYEESGLL ETDEATLDTR KIVEACKAKT DAGGEDAILQ TRTYDLYITY DKYYQTPRLW LFGYDEQRQP LTVEHMYEDI SQDHVKKTVT IENHPHLPPP PMCSVHPCRH AEVMKKIIET VAEGGGELGV HLEHHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product ATG3 Protein
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ATG3 Autophagy Related 3 (ATG3) (AA 1-287) protein (His tag)
ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (GST tag)ATG3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-100) protein (GST tag)ATG3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (Strep Tag)Crystallography grade ATG3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (Strep Tag)Crystallography grade ATG3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314), (full length) protein (His-SUMO Tag)ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
ATG3 Autophagy Related 3 (ATG3) (AA 1-314) protein (His tag)ATG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
ATG3 Autophagy Related 3 (ATG3) protein (Myc-DYKDDDDK Tag)ATG3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- ATG3 (ATG3 Autophagy Related 3 (ATG3))
- Alternative Name
- ATG3 (ATG3 Products)
- Synonyms
- atg3 Protein, im:6910145 Protein, wu:fb15g12 Protein, zgc:64156 Protein, APG3 Protein, APG3-LIKE Protein, APG3L Protein, PC3-96 Protein, 2610016C12Rik Protein, Apg3l Protein, Atg3l Protein, PIG-1 Protein, Pig1 Protein, ATATG3 Protein, K11J9.1 Protein, K11J9_1 Protein, autophagy related 3 Protein, autophagy 3 (APG3) Protein, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy Protein, atg3 Protein, ATG3 Protein, Atg3 Protein
- Gene ID
- 64422
- UniProt
- Q9NT62
- Pathways
- Autophagy
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