CA1 Protein (AA 2-261) (His tag)

Catalog No. ABIN7311452

Product Details

Target: CA1 (Carbonic Anhydrase I (CA1))

Protein Type: Recombinant

Protein Characteristics: AA 2-261

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CA1 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)

Purpose: Recombinant Human CA1 Protein is produced by our E.coli expression system and the target gene encoding Ala2-Phe261 is expressed with a 6His tag at the C-terminus.

Sequence: MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS FLEHHHHHH

Purity: Greater than 95 % as determined by SEC-HPLC and reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a 0.2 μM filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 10 % Glycerol, pH 8.0.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.

Target Details

Target: CA1 (Carbonic Anhydrase I (CA1))

Alternative Name: CA1 (CA1 Products)

Synonyms: CA-I Protein, CAB Protein, Car1 Protein, AW555628 Protein, Ca1 Protein, Car-1 Protein, BG:DS00941.1 Protein, CAH Protein, CG7820 Protein, Dmel\\CG7820 Protein, Gh7 Protein, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 Protein, ATBCA1 Protein, ATSABP3 Protein, BETA CARBONIC ANHYDRASE 1 Protein, F4P13.5 Protein, F4P13_5 Protein, SABP3 Protein, SALICYLIC ACID-BINDING PROTEIN 3 Protein, carbonic anhydrase 1 Protein, CA1 Protein, LOC100135826 Protein, GB15888 Protein, carbonic anhydrase 1 Protein, Carbonic anhydrase 1 Protein, carbonic anhydrase I Protein, carbonic anhydrase Protein, CA1 Protein, Car1 Protein, CAH1 Protein, LOC100135826 Protein, LOC408827 Protein, Tsp_05114 Protein, LOC100153915 Protein, LOC100050192 Protein, Ca1 Protein

Gene ID: 759

UniProt: P00915