P4HB Protein (AA 18-505) (His tag)
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- Target See all P4HB Proteins
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-505
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This P4HB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human P4HB Protein is produced by our E. coli expression system and the target gene encoding Asp18-Lys505 is expressed with a 6His tag at the C-terminus.
- Sequence
- DAPEEEDHVL VLRKSNFAEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA GKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFRN GDTASPKEYT AGREADDIVN WLKKRTGPAA TTLPDGAAAE SLVESSEVAV IGFFKDVESD SAKQFLQAAE AIDDIPFGIT SNSDVFSKYQ LDKDGVVLFK KFDEGRNNFE GEVTKENLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSNFKT AAESFKGKIL FIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESEELTAE RITEFCHRFL EGKIKPHLMS QELPEDWDKQ PVKVLVGKNF EDVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIVIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFK KFLESGGQDG AGDDDDLEDL EEAEEPDMEE DDDQKAVKVD HHHHHH
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product P4HB Protein
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Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) protein (GST tag)
P4HB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 18-508) protein (His tag)P4HB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (Active) protein (His tag)P4HB Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 18-508) protein (His tag)Crystallography grade P4HB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Supplied as a 0.2 μM filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- Alternative Name
- P4HB (P4HB Products)
- Synonyms
- DSI Protein, ERBA2L Protein, GIT Protein, P4Hbeta Protein, PDI Protein, PDIA1 Protein, PHDB Protein, PO4DB Protein, PO4HB Protein, PROHB Protein, PDA2 Protein, PDIP Protein, PDIR Protein, ERp59 Protein, Pdia1 Protein, Thbp Protein, p55 Protein, XPDIp Protein, pdi Protein, 1810041F13Rik Protein, AI661267 Protein, Pdip Protein, Pdipl Protein, p58 Protein, prolyl 4-hydroxylase subunit beta Protein, protein disulfide isomerase family A member 2 Protein, prolyl 4-hydroxylase, beta polypeptide Protein, protein disulfide isomerase family A member 2 L homeolog Protein, protein disulfide isomerase associated 2 Protein, prolyl 4-hydroxylase subunit beta L homeolog Protein, protein disulfide-isomerase Protein, P4HB Protein, PDIA2 Protein, P4hb Protein, pdia2.L Protein, Pdia2 Protein, p4hb.L Protein, LOC8281065 Protein
- Gene ID
- 5034
- UniProt
- P07237
- Pathways
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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