RAD18 Protein (AA 1-495, full length) (His-SUMO Tag)
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- Target See all RAD18 Proteins
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-495, full length
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RAD18 protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MDSLAESRWP PGLAVMKTID DLLRCGICFE YFNIAMIIPQ CSHNYCSLCI RKFLSYKTQC PTCCVTVTEP DLKNNRILDE LVKSLNFARN HLLQFALESP AKSPASSSSK NLAVKVYTPV ASRQSLKQGS RLMDNFLIRE MSGSTSELLI KENKSKFSPQ KEASPAAKTK ETRSVEEIAP DPSEAKRPEP PSTSTLKQVT KVDCPVCGVN IPESHINKHL DSCLSREEKK ESLRSSVHKR KPLPKTVYNL LSDRDLKKKL KEHGLSIQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI VQEIENIEKT RMRLEASKLN ESVMVFTKDQ TEKEIDEIHS KYRKKHKSEF QLLVDQARKG YKKIAGMSQK TVTITKEDES TEKLSSVCMG QEDNMTSVTN HFSQSKLDSP EELEPDREED SSSCIDIQEV LSSSESDSCN SSSSDIIRDL LEEEEAWEAS HKNDLQDTEI SPRQNRRTRA AESAEIEPRN KRNRN
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product RAD18 Protein
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E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) protein (GST tag)
RAD18 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 332-430) protein (GST tag)RAD18 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495) protein (Strep Tag)Crystallography grade RAD18 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-509) protein (Strep Tag)Crystallography grade RAD18 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
E3 ubiquitin-protein ligase RAD18 (RAD18) protein (Myc-DYKDDDDK Tag)RAD18 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
E3 ubiquitin-protein ligase RAD18 (RAD18) (AA 1-495), (full length) protein (His-SUMO Tag)RAD18 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))
- Alternative Name
- RAD18 (RAD18 Products)
- Synonyms
- RNF73 Protein, 2810024C04Rik Protein, Rad18sc Protein, zgc:55577 Protein, RAD18 Protein, DDBDRAFT_0218120 Protein, DDBDRAFT_0304878 Protein, DDB_0218120 Protein, DDB_0304878 Protein, DKFZp469H0810 Protein, RAD18, E3 ubiquitin protein ligase Protein, RAD18 E3 ubiquitin protein ligase Protein, E3 ubiquitin-protein ligase RAD18 Protein, E3 ubiquitin-protein ligase Protein, DNA repair protein and ATPase Protein, C3HC4-type zinc finger-containing protein Protein, RAD18 Protein, Rad18 Protein, rad18 Protein
- Background
- E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
- Molecular Weight
- 72.2 kDa
- UniProt
- Q9NS91
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