ADAR Protein (AA 1-176, partial) (GST tag)

Catalog No. ABIN4976279

Product Details

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Protein Type: Recombinant

Protein Characteristics: AA 1-176, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ADAR protein is labelled with GST tag.

Application: SDS-PAGE (SDS)

Sequence: MNPRQGYSLS GYYTHPFQGY EHRQLRYQQP GPGSSPSSFL LKQIEFLKGQ LPEAPVIGKQ TPSLPPSLPG LRPRFPVLLA SSTRGRQVDI RGVPRGVHLG SQGLQRGFQH PSPRGRSLPQ RGVDCLSSHF QELSIYQDQE QRILKFLEEL GEGKATTAHD LSGKLGTPKK EINRVL

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: ADAR (Adenosine Deaminase, RNA-Specific (ADAR))

Alternative Name: DSRAD (ADAR Products)

Synonyms: red1 Protein, drada Protein, wu:fc22a02 Protein, adar1 Protein, dsRAD Protein, dsRAD-1 Protein, ADAR Protein, ADAR1 Protein, CG12598 Protein, Dmel\\CG12598 Protein, EG:BACN35H14.1 Protein, adar Protein, adr Protein, cg12598 Protein, dADAR Protein, dAdar Protein, hypnos-2 Protein, NV18763 Protein, AGS6 Protein, DRADA Protein, DSH Protein, DSRAD Protein, G1P1 Protein, IFI-4 Protein, IFI4 Protein, K88DSRBP Protein, P136 Protein, AV242451 Protein, Adar1 Protein, mZaADAR Protein, adenosine deaminase, RNA-specific Protein, adenosine deaminase, RNA-specific S homeolog Protein, adenosine deaminase, RNA specific Protein, Adenosine deaminase acting on RNA Protein, adenosine deaminase acting on RNA Protein, double-stranded RNA-specific editase 1 Protein, adar Protein, adar.S Protein, ADAR Protein, Adar Protein, CpipJ_CPIJ011849 Protein, LOC100114127 Protein

Background: Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins, pre-mRNA splicing by altering splice site recognition sequences, RNA stability by changing sequences involved in nuclease recognition, genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication, and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Its viral RNA substrates include: hepatitis C virus (HCV), vesicular stomatitis virus (VSV), measles virus (MV), hepatitis delta virus (HDV), and human immunodeficiency virus type 1 (HIV-1). Exhibits either a proviral (HDV, MV, VSV and HIV-1) or an antiviral effect (HCV) and this can be editing-dependent (HDV and HCV), editing-independent (VSV and MV) or both (HIV-1). Impairs HCV replication via RNA editing at multiple sites. Enhances the replication of MV, VSV and HIV-1 through an editing-independent mechanism via suppression of EIF2AK2/PKR activation and function. Stimulates both the release and infectivity of HIV-1 viral particles by an editing-dependent mechanism where it associates with viral RNAs and edits adenosines in the 5'UTR and the Rev and Tat coding sequence. Can enhance viral replication of HDV via A-to-I editing at a site designated as amber/W, thereby changing an UAG amber stop codon to an UIG tryptophan (W) codon that permits synthesis of the large delta antigen (L-HDAg) which has a key role in the assbly of viral particles. However, high levels of ADAR1 inhibit HDV replication

Molecular Weight: 47 kDa

UniProt: P55265

Pathways: Protein targeting to Nucleus