HUS1 Protein (AA 2-280, partial) (His-SUMO Tag)
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- Target See all HUS1 Proteins
- HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))
- Protein Type
- Recombinant
- Protein Characteristics
- partial, AA 2-280
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HUS1 protein is labelled with His-SUMO Tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- KFRAKIVDGA CLNHFTRISN MIAKLAKTCT LRISPDKLNF ILCDKLANGG VSMWCELEQE NFFNEFQMEG VSAENNEIYL ELTSENLSRA LKTAQNARAL KIKLTNKHFP CLTVSVELLS MSSSSRIVTH DIPIKVIPRK LWKDLQEPVV PDPDVSIYLP VLKTMKSVVE KMKNISNHLV IEANLDGELN LKIETELVCV TTHFKDLGNP PLASESTHED RNVEHMAEVH IDIRKLLQFL AGQQVNPTKA LCNIVNNKMV HFDLLHEDVS LQYFIPALS
- Purification
- SDS-PAGE
- Purity
- > 90 %
- Top Product
- Discover our top product HUS1 Protein
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HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (His tag)
HUS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (GST tag)HUS1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (Strep Tag)Crystallography grade HUS1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 1-280) protein (Strep Tag)Crystallography grade HUS1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) (AA 2-280), (partial) protein (His-SUMO Tag)HUS1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
HUS1 Checkpoint Homolog (S. Pombe) (HUS1) protein (Myc-DYKDDDDK Tag)HUS1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1-2 mg/mL
- Buffer
- 20 mM Tris-HCl based buffer, pH 8.0
- Storage
- -80 °C,4 °C,-20 °C
- Storage Comment
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- Target
- HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))
- Alternative Name
- HUS1 (HUS1 Products)
- Synonyms
- hHUS1 Protein, mHus1 Protein, XHus1 Protein, hus1 Protein, HUS1 checkpoint clamp component Protein, checkpoint clamp complex protein Hus1 Protein, HUS1 checkpoint clamp component L homeolog Protein, HUS1 Protein, Hus1 Protein, hus1 Protein, hus1.L Protein
- Background
- Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-tplate and stabilizes POLB to those sites where LP-BER proceeds, endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths, and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase.
- Molecular Weight
- 47.5 kDa
- UniProt
- O60921
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