SMARCB1 Protein (AA 2-376, partial) (His tag)

Catalog No. ABIN4976333

Product Details

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Protein Type: Recombinant

Protein Characteristics: AA 2-376, partial

Origin: Human

Source: Yeast

Purification tag / Conjugate: This SMARCB1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: MMMALSKTFG QKPVKFQLED DGEFYMIGSE VGNYLRMFRG SLYKRYPSLW RRLATVEERK KIVASSHGKK TKPNTKDHGY TTLATSVTLL KASEVEEILD GNDEKYKAVS ISTEPPTYLR EQKAKRNSQW VPTLPNSSHH LDAVPCSTTI NRNRMGRDKK RTFPLCFDDH DPAVIHENAS QPEVLVPIRL DMEIDGQKLR DAFTWNMNEK LMTPEMFSEI LCDDLDLNPL TFVPAIASAI RQQIESYPTD SILEDQSDQR VIIKLNIHVG NISLVDQFEW DMSEKENSPE KFALKLCSEL GLGGEFVTTI AYSIRGQLSW HQKTYAFSEN PLPTVEIAIR NTGDADQWCP LLETLTDAEM EKKIRDQDRN TRRMR

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Alternative Name: SNF5 (SMARCB1 Products)

Synonyms: SMARCB1 Protein, BAF47 Protein, INI1 Protein, MRD15 Protein, RDT Protein, RTPS1 Protein, SNF5 Protein, SNF5L1 Protein, Sfh1p Protein, Snr1 Protein, hSNFS Protein, AU020204 Protein, Baf47 Protein, Ini1 Protein, SNF5/INI1 Protein, Snf5 Protein, fc51b01 Protein, smarcb1 Protein, wu:fc51b01 Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 S homeolog Protein, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1b Protein, SMARCB1 Protein, Smarcb1 Protein, smarcb1.S Protein, smarcb1b Protein

Background: Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-rodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the rodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin rodeling complex (npBAF complex) and the neuron-specific chromatin rodeling complex (nBAF complex). During neural development a switch from a st/progenitor to a post-mitotic chromatin rodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural st/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural st cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth . Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1.

Molecular Weight: 45 kDa

UniProt: Q12824