EGLN3 Protein (AA 2-239) (His tag)

Catalog No. ABIN4976805

Product Details

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Protein Type: Recombinant

Protein Characteristics: AA 2-239

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EGLN3 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Sequence: PLGHIMRLDL EKIALEYIVP CLHEVGFCYL DNFLGEVVGD CVLERVKQLH YNGALRDGQL AGPCAGVSKR HLRGDQITWI GGNEEGCEAI NFLLSLIDRL VLYCGSRLGK YYVKERSKAM VACYPGNGTG YVRHVDNPNG DGRCITCIYY LNKNWDAKLH GGVLRIFPEG KSFVADVEPI FDRLLFFWSD RRNPHEVQPS YATRYAMTVW YFDAEERAEA KKKFRNLTRK TESALAKD

Purification: SDS-PAGE

Purity: > 90 %

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Storage: -80 °C,4 °C,-20 °C

Storage Comment: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3 (EGLN3 Products)

Synonyms: zgc:77019 Protein, wu:fj78a08 Protein, EGLN3 Protein, phd3 Protein, PHD-3 Protein, PHD3 Protein, SM-20 Protein, 2610021G09Rik Protein, AI505553 Protein, AI648162 Protein, Hif-p4h-3 Protein, Phd3 Protein, HIFP4H3 Protein, HIFPH3 Protein, egl-9 family hypoxia-inducible factor 3 Protein, egl-9 family hypoxia inducible factor 3 Protein, egl-9 family hypoxia-inducible factor 3 L homeolog Protein, egln3 Protein, EGLN3 Protein, egln3.L Protein, Egln3 Protein

Background: Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway . Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. ELGN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Target proteins are preferencially recognized via a LXXLAP motif.1 Publication

Molecular Weight: 31.2 kDa

UniProt: Q91UZ4

Pathways: Positive Regulation of Endopeptidase Activity