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Aminoacylase 1 Protein (ACY1) (DYKDDDDK Tag)

ACY1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Catalog No. ABIN5505551
  • Target See all Aminoacylase 1 (ACY1) Proteins
    Aminoacylase 1 (ACY1)
    Protein Type
    Recombinant
    Origin
    • 5
    • 3
    • 1
    • 1
    Human
    Source
    • 4
    • 2
    • 2
    • 1
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This Aminoacylase 1 protein is labelled with DYKDDDDK Tag.
    Application
    Antibody Production (AbP), Standard (STD)
    Characteristics
    • Recombinant human Purified recombinant protein of Human aminoacylase 1 (ACY1), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9, 20 μg (full length, C-term DDK tag, transcript variant 1) protein expressed in Sf9 cells.
    • Produced with end-sequenced ORF clone
    Purification
    Purified
    Purity
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Top Product
    Discover our top product ACY1 Protein
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    50 μg/mL
    Buffer
    50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    Aminoacylase 1 (ACY1)
    Alternative Name
    aminoacylase 1 (ACY1 Products)
    Synonyms
    si:zfos-226g1.1 Protein, zgc:55605 Protein, zgc:76955 Protein, acy1 Protein, acy1d Protein, acylase Protein, ACY1 Protein, ACY-1 Protein, ACY1D Protein, 1110014J22Rik Protein, Acy-1 Protein, Acy1a Protein, Acy1b Protein, aminoacylase 1 Protein, aminoacylase 1, gene 1 S homeolog Protein, aminoacylase 1, gene 1 Protein, ACY1 Protein, acy1 Protein, acy1.1.S Protein, acy1.1 Protein, Acy1 Protein
    Background
    This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18.
    Molecular Weight
    45.7 kDa
    NCBI Accession
    NP_000657
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