CRISPR-Cas9 protein (His tag)
Origin: Streptococcus pyogenes
Host: Escherichia coli (E. coli)
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- Target
- CRISPR-Cas9
- Protein Type
- Recombinant
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Origin
- Streptococcus pyogenes
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human CAS9 (N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 300 mM NaCl,10 mM Tris,0.1 mM EDTA,1 mM DTT, pH 7.4, 50 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- CRISPR-Cas9
- Alternative Name
- CAS9
- Molecular Weight
- 160 kDa
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