GZMM Protein (AA 26-257) (His tag)
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- Target See all GZMM Proteins
- GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))
- Biological Activity
- Active
- Protein Characteristics
- AA 26-257
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GZMM protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 90 %
- Biological Activity Comment
- GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets α-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2x107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs β-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin. Normal Heated 0 4 4 GZMM (μM) Ezrin Actin Figure 1. GZMM cleaves Ezrin
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Granzyme M (Lymphocyte Met-Ase 1) (GZMM) (AA 26-257) protein (His tag)Crystallography grade GZMM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Granzyme M (Lymphocyte Met-Ase 1) (GZMM) (AA 26-257) (Active) protein (His tag)GZMM Origin: Human Host: Escherichia coli (E. coli) > 90 % AcA, CC Active
Granzyme M (Lymphocyte Met-Ase 1) (GZMM) proteinGZMM Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Application Notes
- Isoelectric Point: 10.3
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- GZMM (Granzyme M (Lymphocyte Met-Ase 1) (GZMM))
- Alternative Name
- Granzyme M (GZMM) (GZMM Products)
- Synonyms
- LMET1 Protein, MET1 Protein, Lmet1 Protein, MMET-1 Protein, granzyme M Protein, granzyme M (lymphocyte met-ase 1) Protein, LOC100136381 Protein, GZMM Protein, Gzmm Protein
- Background
- Alternative Names: GZM-M, LMET1, MET1, Hu-Met-1, Met-ase, Lymphocyte Met Ase 1, Met-1 serine protease, Natural killer cell granular protease
- Molecular Weight
- 26kDa
- UniProt
- P51124
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