LOX Protein (AA 213-417) (His tag,GST tag)

Catalog No. ABIN5665872

Product Details

Target: LOX (Lysyl Oxidase (LOX))

Biological Activity: Active

Protein Characteristics: AA 213-417

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LOX protein is labelled with His tag,GST tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His and GST Tag

Purity: > 90 %

Biological Activity Comment: Lysyl oxidase (LOX) also known as protein-lysine 6-oxidase is an extracellular copper-dependent enzyme that catalyzes formation of aldehydes from lysine residues in collagen and elastin precursors. Its catalytic activity depends upon both its copper cofactor and a unique carbonyl cofactor and has been shown to extend to a variety of basic globular proteins, including histone H1. LOX plays a major role in connective tissue development and may also be important in neurological function. Lysyl oxidase has also proven crucial to the development of the respiratory system and the skin, the commitment step of adipocyte, and the formation of pluripotent stem cells during development. Its absence may lead to defects in the transforming growth factor beta superfamily of proteins, which control cell growth and differentiation. Besides, Elastin (ELN) has been identified as an interactor of LOX, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LOX and recombinant human ELN. Briefly, LOX were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ELN-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LOX pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of LOX and ELN was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of LOX with ELN.

Application Details

Application Notes: Isoelectric Point: 6.2

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: LOX (Lysyl Oxidase (LOX))

Abstract: LOX Products

Synonyms: LOX Protein, Xlox Protein, MGC146507 Protein, lox Protein, zgc:77447 Protein, AI893619 Protein, TSC-160 Protein, H-rev142 Protein, Rrg1 Protein, lysyl oxidase Protein, lysyl oxidase a Protein, lysyl oxidase L homeolog Protein, LOX Protein, lox Protein, loxa Protein, lox.L Protein, Lox Protein

Background: Alternative Names: Protein-Lysine 6-Oxidase

Molecular Weight: 53kDa

UniProt: P28300

Pathways: SARS-CoV-2 Protein Interactome