INHBA Protein

Name: INHBA

Source: Nicotiana benthamiana

Origin (Gene): Human

Catalog no.: ABIN573784

Additional Information

Characteristics: Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody Activin A.
Molecular formula: C600H911N173O174S13.
Isoelectric Point: 7,27.
Extinction coefficient: E 0.1% (1g/L) = 1.27 (A 280 nm).

Gene ID: 3624, 147290

Format: Lyophilized

Endotoxin Level: <0.04 EU/ug protein (LAL method)

Description: Activins are homodimers or heterodimers of the various beta subunit isoforms, belonging to the TGFbeta family. Mature Activin A has two 116 amino acids residues betaA subunits (betaA-betaA). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Cells known to express Activin A include fibroblasts, endothelial cells, hepatocytes, vascular smooth muscle cells, macrophages, keratinocytes, osteoclasts, bone marrow monocytes, prostatic epithelium, neurons, chondrocytes, osteoblasts, Leydig cells, Sertoli cells, and ovarian granulosa cells. As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B. The biological activity of Activin A can be neutralized by inhibins and by the diffusible TGF-B antagonist, Follistatin.

Molecular Weight: Recombinant human Activin A is a 27.4 kDa disulphide-linked homodimers of two beta-A chains, each containing 116 amino residues.

Comments:

Synonyms: Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein

Application Details

Concentration: 50 ng/µl

Purity: > 97%, The protein was resolved by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Purification: This product contains no animal–derived components or impurities.
Produced by transient expression of Activin A in non-transgenic plants. Recombinant human Activin A contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Buffer: Lyophilized from a Tris HCl 0.05M buffer at pH 7.4.

Storage: This lyophilized preparation is stable at 2-8º C for short term, long storage it should be kept at -20ºC. Reconstituted protein should be stored in working aliquots at –20°. Repeated freezing and thawing is not recommended.

Restrictions: For Research Use only