Follistatin (FST) (Active) Protein

Name: Follistatin (FST)

Synonyms: CG12955, CG12956, Fol1, dFS, dFol1, Dmel\CG33466, CG33466, FS, xfs, MGC79635, FST, fst, LOC100033825, Fst, AL033346, FOL1, Fst-288, RATFOL1

Protein Type: Recombinant

Biological Activity: Active

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Catalog no.: ABIN593426

Additional Information

Gene ID: 10468

UniProt: P19883

Endotoxin Level: <0.1 ng/µg

Description: Follistatin belongs to a group of structurally-diverse diffusible proteins that binds to TGF-beta ligands and inhibit their activity by hindering access to signaling receptors. This naturally occurring antagonist binds to Activin, BMP-2,-4,-6,-7, Myostatin, GDF-11, and TGF-beta1. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. Recombinant human Follistatin is a 31.5 kDa protein containing 288 amino acids including 36 cysteine residues.
Synonyms: Human Follistatin, Follistatin, h-Follistatin, rh-Follistatin, recombinant human Follistatin, recombinant Follistatin, Follistatin, FST, FS, Activin-binding protein

Characteristics: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED₅₀ is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A.

Specificity: Determined by its ability to neutralize Activin A inhibitory effect of murine MPC-11 cells. The expected ED50 is 0.1-0.4 µg/ml in the presence of 7.5 ng/ml Activin A. Results: 0.1-0.4 µg/ml.

Molecular Weight: 31.5 kDa

Application Details

Handling Advice: Centrifuge the vial prior to opening.

Purity: SDS-PAGE: >=95%<0.1 ng/µg

Buffer: Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 75 mM NaCl

Storage: -20°C.
Stability: 12 months

Research Area: Stem Cells, Proliferation, Signaling, Enzymes

Restrictions: For Research Use only

Publications

Product: Rose, Mattis, Rindt et al.: "Delivery of recombinant follistatin lessens disease severity in a mouse model of spinal muscular atrophy." in: Human molecular genetics, Vol. 18, Issue 6, pp. 997-1005, 2009 (PubMed).

Umezu, Yamanouchi, Iida et al.: "Follistatin-like-1, a diffusible mesenchymal factor determines the fate of epithelium." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 10, pp. 4601-6, 2010 (PubMed).

Li, Shimono, Norioka et al.: "Activin A binds to perlecan through its pro-region that has heparin/heparan sulfate binding activity." in: The Journal of biological chemistry, Vol. 285, Issue 47, pp. 36645-55, 2010 (PubMed).