Leptin Receptor (LEPR) (AA 1-839) (Active) protein (His tag)

Details for Product No. ABIN593497, Supplier: Log in to see
Protein Name
  • obr
  • cd295
  • ob-rb
  • CD295
  • LEP-R
  • LEPRD
  • OB-R
  • OBR
  • ObRb
  • LEPROT
  • Leprb
  • Modb1
  • OB-RGRP
  • Obr
  • db
  • diabetes
  • obese-like
  • obl
  • Ob-R
  • Ob-RM
  • Fa
  • LEPR
  • Obrgrp
  • VPS55
  • leptin receptor
  • leptin receptor overlapping transcript
  • Leptin receptor-like
  • leptin receptor-like
  • lepr
  • LEPR
  • Lepr
  • Leprot
  • LOC100351924
  • LOC100069504
Protein Characteristics
AA 1-839
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Origin
Human
17
4
1
Source
Human
6
6
2
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This Leptin Receptor protein is labelled with His tag.
Application
High Pressure Liquid Chromatography (HPLC), SDS-PAGE (SDS)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Characteristics Molecule Information: 95.0 kDa (predicted) 130-140 kDa on a gel.

Sequence Information: A DNA sequence encoding the extracellular domain (Met 1 - Asp 839) of human leptin receptor (NP_002294.2) was expressed and fused with a C-terminal polyhistidine tag. Predicted NT sequence is Phe 22.
Purity ≥95 % by SDS-PAGE
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032 - 4.0 μg/ml.
Background Leptin receptor (LEPR), also known as OB-R and B219, is a single transmembrane-domain receptor of the cytokine receptor family. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is expressed at high levels in hematopoietic stem cells, lymphohematopoietic cell lines, as well as adult reproductive organs. Several isoforms of LEPR have been identified, and LEPR structurally contains two hemopoietin receptor domains, a fibronectin type III domain and a WSXWS domain within the extracellular region. Interaction of leptin and leptin receptor is crucial for bodyweight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.

Alternative Names: OB Protein, Obesity Protein, OBS, Obesity factor, HuB219, OB receptor, CD_antigen=CD295
Molecular Weight 95.0 kDa
Gene ID 3952
UniProt P41159
Research Area Cardiovascular, Fatty Acids, Hormones, Receptors
Pathways JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Centrifuge the vial prior to opening. The lyophilized Leptin Binding Domain is very soluble in H₂O and most aqueous buffers below and above the isoelectric point (~ 5.9).
Buffer Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Leptin Receptor (LEPR) (AA 1-839) (Active) protein (His tag) (ABIN593497) Leptin Receptor (LEPR) (AA 1-839) (Active) protein (His tag)
Product cited in: Sato, Schehr, Ikegami: "Leptin does not influence surfactant synthesis in fetal sheep and mice lungs." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 3, pp. L498-505, 2011 (PubMed).

Did you look for something else?