Adiponectin (ADIPOQ) / Acrp30 Protein

Name: Adiponectin (ADIPOQ) / Acrp30

Protein Type: Recombinant

Source: HEK

Origin (Gene): Human

Application: ELISA, Western Blotting (WB), Cell Culture (CC)

Catalog no.: ABIN620503

Additional Information

Characteristics: Recombinant Human Adiponectin (ADIPOQ) / Acrp30, Glycosylated, HEK-expressed

Sequence: ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTND

Description: The Acrp30 Human Recombinant is fused with FLAG tag. Introduction: Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family, it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. APM-1 forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Synonyms: Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Application Details

Purity: Greater than 98% as determined by HPLC and SDS-PAGE. Activity in vitroIn vitro gluconeogenesis assay in primary hepatocytes was performed, showing the human Adiponectin derived from mammalian cells can inhibit glucose production. The ED50 was ~6 µg/ml.

Buffer: Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer.Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.

Storage: For long term, store lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time, it does not show any change after two weeks at 4°C. The lyophilized protein remains stable for 24 months when stored at -20°C.

Restrictions: For Research Use only