PHOSPHO1 Protein (His tag)
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- Target See all PHOSPHO1 Proteins
- PHOSPHO1 (Phosphatase, Orphan 1 (PHOSPHO1))
- Protein Type
- Recombinant
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Origin
- Human
- Host
- Please inquire
- Purification tag / Conjugate
- This PHOSPHO1 protein is labelled with His tag.
- Sequence
- MRGSHHHHHH GMASMSGCFP VSGLRCLSRD GRMAAQGAPR FLLTFDFDET IVDENSDDSI VRAAPGQRLP ESLRATYREG FYNEYMQRVF KYLGEQGVRP RDLSAIYEAI PLSPGMSDLL QFVAKQGACF EVILISDANT FGVESSLRAA GHHSLFRRIL SNPSGPDARG LLALRPFHTH SCARCPANMC KHKVLSDYLR ERAHDGVHFE RLFYVGDGAN DFCPMGLLAG GDVAFPRRGY PMHRLIQEAQ KAEPSSFRAS VVPWETAADV RLHLQQVLKS C.
- Characteristics
- Recombinant Human Phosphoethanolamine/Phosphocholine Phosphatase
- Purity
- > 95 % as determined by SDS-PAGE.
- Sterility
- Sterile filtered
- Top Product
- Discover our top product PHOSPHO1 Protein
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Phosphatase, Orphan 1 (PHOSPHO1) (AA 168-266) protein (GST tag)
PHOSPHO1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phosphatase, Orphan 1 (PHOSPHO1) (AA 1-267) protein (Strep Tag)Crystallography grade PHOSPHO1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Phosphatase, Orphan 1 (PHOSPHO1) (AA 1-267) protein (Strep Tag)Crystallography grade PHOSPHO1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Phosphatase, Orphan 1 (PHOSPHO1) protein (Myc-DYKDDDDK Tag)PHOSPHO1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- The sterile filtered concentrated (0.5 mg/ml) protein solution was lyophilized with 30mM acetate buffer pH-4.Add 0.1M acetate buffer pH4 to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely.
- Storage
- -20 °C
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- Target
- PHOSPHO1 (Phosphatase, Orphan 1 (PHOSPHO1))
- Alternative Name
- Phosphoethanolamine/Phosphocholine Phosphatase (PHOSPHO1 Products)
- Synonyms
- PHOSPHO1 Protein, sb:cb839 Protein, zgc:92423 Protein, D11Moh36 Protein, Phospo1 Protein, phosphoethanolamine/phosphocholine phosphatase Protein, phosphatase, orphan 1 Protein, phosphoethanolamine/phosphocholine phosphatase 1 Protein, PHOSPHO1 Protein, phospho1 Protein, Phospho1 Protein
- Background
- Human Phospho1 Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 295 AA and having a molecular mass of 31.3 kDa. The Human Phospho1 is fused to His tag at N-Terminus. Human Phosphocholine Phosphatase is purified by proprietary chromatographic techniques. Introduction: PHOSPHO1 is involved in mineralization process and plays a role in bone and cartilage matrix mineralization. PHOSPHO1 is expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix. Orphan-1 is collagen type -2 is specific for cartilaginous tissues. Orphan1 is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Phosphoethanolamine (2-O3POCH2CH2NH3) is a key intermediate in the formation of cephalins, it is formed in liver and brain by phosphorylation of ethanolamine. PHOSPHO2 and PHOSPHO1 suggest subtle differences in the charge distributions around the putative substrate entry site and in the location of potential H-bond donors. PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities PHOSPHO1 is a phosphatase enzyme for which expression is upregulated in mineralizing cells. PHOSPHO1 has been implicated in the generation of Pi for matrix mineralization, a process central to skeletal development. PHOSPHO1 is a member of the haloacid dehalogenase (HAD) superfamily of Mg2+-dependent hydrolases. PHOSPHO1 exhibits high specific activities toward phosphoethanolamine (PEA) and phosphocholine (PCho). Synonyms: Phosphoethanolamine/phosphocholine phosphatase, Phosphatase, Orphan 1, EC 3.1.3.75, Phospho1.
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