Cytokeratin 8 (KRT8) Protein

Name: Cytokeratin 8 (KRT8)

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, K18, CYK18, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, KRT2-8

Protein Type: Recombinant

Origin (Gene): Human

Catalog no.: ABIN621237

Additional Information

Characteristics: Recombinant Human Cytokeratin 8

Format: Lyophilized

Reconstitution: to Filaments Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer . Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

Description: Cytokeratin 8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 53,532 Dalton. The CK-8 is purified by proprietary chromatographic techniques. Introduction: Keratin 8 and 18 (K8/18) are the major components of intermediate filament (IF) proteins of simple or single-layered epithelia. Synonyms: Keratin type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, KRT8, CYK8, KO, CK8, K2C8, CARD2.

Application Details

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Buffer: The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride.It is recommended to reconstitute the lyophilized CK-8 in sterile 18M -cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage: Lyophilized CK-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CK-8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Restrictions: For Research Use only