HMGB1 Protein (His tag)

Catalog No. ABIN623045

Product Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Origin: Human

Host: Please inquire

Purification tag / Conjugate: This HMGB1 protein is labelled with His tag.

Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDELEHHHH HH

Characteristics: Recombinant Human High-Mobility Group Box 1

Purity: > 95.0 % as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: The HMG1 (1 mg/ml) was lyophilized after extensive dialyses against 1x PBS pH-7.4.It is recommended to reconstitute the lyophilized HMGB1 in sterile 18M -cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Storage: -20 °C

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High-Mobility Group Box 1 (HMGB1 Products)

Synonyms: HMG1 Protein, HMG3 Protein, SBP-1 Protein, DEF Protein, HMG-1 Protein, Hmg1 Protein, amphoterin Protein, p30 Protein, hmgb1 Protein, ik:tdsubc_1a5 Protein, wu:fb23c02 Protein, xx:tdsubc_1a5 Protein, zgc:56110 Protein, zgc:77104 Protein, hmg-1 Protein, hmg3 Protein, sbp-1 Protein, hmg1 Protein, HMGB1 Protein, Ac2-008 Protein, high mobility group box 1 Protein, high-mobility group box 1 Protein, high mobility group box 1a Protein, high mobility group box 1 L homeolog Protein, high mobility group protein B1 Protein, HMGB1 Protein, Hmgb1 Protein, hmgb1 Protein, hmgb1a Protein, hmgb1.L Protein, LOC100359149 Protein

Background: HMG1 Human Recombinant fused with 6X His tag produced in E. coli is a single, non-glycosylated, polypeptide chain containing 223 AA and having a molecular mass of 26 kDa. The HMGB-1 is purified by proprietary chromatographic techniques. Introduction: High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 AA (AA) single chain polypeptide containing three domains: two N-terminal globular, 70 AA positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 AA C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. Synonyms: HMG1, HMG3, SBP-1, Amphoterin, HMGB1, High-Mobility Group Box 1.

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome