Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor) (HGF) Protein

Name: Hepatocyte Growth Factor (Hepapoietin A; Scatter Factor) (HGF)

Synonyms: SF, HGFB, HPTA, F-TCF, DFNB39, NK1, NK2, HGF/SF, SF/HGF, C230052L06Rik, HGF, CACNA2D1

Protein Type: Recombinant

Source: Baculovirus/Sf9

Origin (Gene): Human

Catalog no.: ABIN623094

Additional Information

Characteristics: Recombinant Human Hepatocyte Growth Factor, Baculovirus/Sf9-expressed

Sequence: Agrees with the sequence of native human HGF. Protein Content Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard. Stability Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw

Format: Lyophilized

Application Details

Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Buffer: The sterile protein powder (1 mg/ml) is lyophilized from a solution containing 50mM Acetic Acid.It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M -cm H 2 O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage: Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Restrictions: For Research Use only