Collagen, Type I (COL1) Protein

Name: Collagen, Type I (COL1)

Protein Type: Native

Origin (Gene): Human

Catalog no.: ABIN623464

Additional Information

Characteristics: Native Human Collagen I

Format: Lyophilized

Reconstitution: Use 0.05-0.5 M acetic acid, pH 2.5 at 4ºC.

Specificity: Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis

Molecular Weight: 300 kDa

Application Details

Reagent Preparation: Collagen from washed dissected tissue was dissolved in acetic acid after mild pepsin treatment. Collagen type I was then purified using differential salt precipitation.

Purity: >90%

Purification: Purified protein from human placenta

Buffer: Phosphate buffered saline

Storage: Store at -20°C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of dispatch.

Restrictions: For Research Use only