MGMT Protein (AA 1-207) (His tag)
-
- Target See all MGMT Proteins
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-207
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MGMT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- MGMT, 1-207aa, Human, His-tagged, Recombinant, E.coli
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product MGMT Protein
-
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)
MGMT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-100) protein (GST tag)MGMT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (Strep Tag)Crystallography grade MGMT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-211) protein (Strep Tag)Crystallography grade MGMT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) protein (Myc-DYKDDDDK Tag)MGMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol
- Storage
- 4 °C
-
- Target
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- Alternative Name
- MGMT (MGMT Products)
- Synonyms
- AGT Protein, AI267024 Protein, Agat Protein, O-6-methylguanine-DNA methyltransferase Protein, MGMT Protein, Mgmt Protein
- Background
- O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E.coli and was purified by conventional chromatography techniques. Synonyms: O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase. NCBI no.: NP_002403
- Molecular Weight
- 23.8 kDa (227 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
- Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
-
