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Dihydrofolate Reductase Protein (DHFR) (AA 1-187) (His tag)

DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Catalog No. ABIN666887
  • Target See all Dihydrofolate Reductase (DHFR) Proteins
    Dihydrofolate Reductase (DHFR)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-187
    Origin
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 14
    • 5
    • 2
    • 2
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This Dihydrofolate Reductase protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Characteristics
    DHFR, 1-187 aa, Human, His-tagged, Recombinant, E.coli
    Purity
    > 95 % by SDS - PAGE
    Top Product
    Discover our top product DHFR Protein
  • Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/ml (determined by Bradford assay)
    Buffer
    Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol
    Storage
    4 °C
  • Target
    Dihydrofolate Reductase (DHFR)
    Alternative Name
    DHFR (DHFR Products)
    Synonyms
    DHFR Protein, F10_orf160 Protein, ECK0049 Protein, JW0047 Protein, tmrA Protein, cb595 Protein, zgc:86637 Protein, DDBDRAFT_0187132 Protein, DDBDRAFT_0230113 Protein, DDB_0187132 Protein, DDB_0230113 Protein, CG14887 Protein, Dmel\\CG14887 Protein, dhfr Protein, DHFRP1 Protein, DYR Protein, 8430436I03Rik Protein, AA607882 Protein, AI662710 Protein, AW555094 Protein, Dhfr1 Protein, confers resistance to sulfonamides Protein, dihydrofolate reductase Protein, dihydrofolate reductase DhfR Protein, Dihydrofolate reductase Protein, dhfr Protein, DHFR Protein, dhfR Protein, folA Protein, dfrA Protein, LOC707382 Protein, CNJ01940 Protein, DDB_G0286755 Protein, Dhfr Protein, Mesil_0096 Protein, Arch_0408 Protein, Fbal_3367 Protein, Ilyop_0482 Protein, Lbys_2007 Protein, Riean_0733 Protein, Mrub_1855 Protein, Poras_0766 Protein, Halhy_3552 Protein, Mesop_3614 Protein, LOC609048 Protein
    Background
    DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Dihydrofolate reductase, EC 1.5.1.3. NCBI no.: NP_000782.1
    Molecular Weight
    23.6 kDa (207aa), confirmed by MALDI-TOF.
    Pathways
    Mitotic G1-G1/S Phases
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