DFFA Protein (AA 1-331) (His tag)

Catalog No. ABIN667128

Product Details

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Protein Type: Recombinant

Protein Characteristics: AA 1-331

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DFFA protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: DFFA, 1-331 aa, Human, His-tagged, Recombinant, E.coli

Purity: > 90 % by SDS-PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.

Storage: 4 °C

Target Details

Target: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Alternative Name: DFFA (DFFA Products)

Synonyms: DFF-45 Protein, DFF1 Protein, ICAD Protein, A330085O09Rik Protein, DFF35 Protein, Dff45 Protein, ICAD-L Protein, ICAD-S Protein, DFFA Protein, zgc:92229 Protein, DNA fragmentation factor subunit alpha Protein, DNA fragmentation factor, alpha subunit Protein, DNA fragmentation factor, 45kDa, alpha polypeptide Protein, DNA fragmentation factor, alpha polypeptide Protein, DFFA Protein, Dffa Protein, dffa Protein

Background: DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: FF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, A330085O09Rik, ICAD L, ICAD S, DFF 1, Caspase activated deoxyribonuclease inhibitor short form, DFF 45, DFF alpha, DFF35, DFF45, DFFA, Caspase-activated deoxyribonuclease inhibitor short form, Dffa DNA fragmentation factor, alpha subunit, DNA fragmentation factor 45 kDa subunit, DNA Fragmentation Factor Alpha Subunit, DNA fragmentation factor subunit alpha, DNA fragmentation factor, 45 kD, alpha subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, RP23 121D17.3, DNA fragmentation factor, alpha subunit, DNAation factor 45 kDa subunit, H13, Inhibitor of CAD, Inhibitor of Caspase Activated DNase, MGC143066, OTTHUMP00000001903, OTTHUMP00000001904,. NCBI no.: AAH07721

Molecular Weight: 38.7 kDa (351aa), confirmed by MALDI-TOF.

Pathways: Apoptosis, Caspase Cascade in Apoptosis