SAE1 Protein (AA 1-346) (His tag)
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- Target See all SAE1 Proteins
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-346
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SAE1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- SAE1, 1-346aa, Human, His-T7 tag, E.coli
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product SAE1 Protein
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SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (His tag)
SAE1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (GST tag)SAE1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (Strep Tag)Crystallography grade SAE1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 2-346) protein (His tag)Crystallography grade SAE1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-350) protein (Strep Tag)Crystallography grade SAE1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 2-350) protein (His tag)Crystallography grade SAE1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SAE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SAE1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
- Storage
- 4 °C
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- Target
- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
- Alternative Name
- SAE1 (SAE1 Products)
- Synonyms
- AOS1 Protein, HSPC140 Protein, SUA1 Protein, UBLE1A Protein, 2400010M20Rik Protein, 2610044L12Rik Protein, AL033372 Protein, AW743391 Protein, D7Ertd177e Protein, Sua1 Protein, Uble1a Protein, Aos1p Protein, Sua1p Protein, aos Protein, sae2a Protein, uble1a Protein, wu:fa28b04 Protein, zgc:86633 Protein, SUMO1 activating enzyme subunit 1 Protein, SUMO1 activating enzyme subunit 1 L homeolog Protein, SAE1 Protein, Sae1 Protein, sae1.L Protein, sae1 Protein
- Background
- SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. This protein can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: AOS1, HSPC140, SUA1, UBLE1A, SUMO1 activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, AOS 1, HSPC 140, SUA 1, UBLE 1A, UBL E1A, SAE 1, Ubiquitin like protein SUMO 1 activating enzyme. NCBI no.: NP_005491
- Molecular Weight
- 42.2 kDa (378aa) confirmed by MALDI-TOF
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