MCEE Protein (AA 37-176) (His tag)

Catalog No. ABIN667242

Product Details

Target: MCEE (Methylmalonyl CoA Epimerase (MCEE))

Protein Type: Recombinant

Protein Characteristics: AA 37-176

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MCEE protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: MCEE, 37-176aa, Human, His tag, E.coli

Purity: > 90 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 10% glycerol

Storage: 4 °C

Target Details

Target: MCEE (Methylmalonyl CoA Epimerase (MCEE))

Alternative Name: MCEE (MCEE Products)

Synonyms: MGC89112 Protein, zgc:112343 Protein, MGC116480 Protein, GLOD2 Protein, 1110007A04Rik Protein, methylmalonyl-CoA epimerase Protein, methylmalonyl CoA epimerase Protein, methylmalonyl-CoA epimerase S homeolog Protein, MCEE Protein, mcee Protein, mcee.S Protein, Mcee Protein

Background: MCEE catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids, odd chain-length fatty acids, and other metabolites. This protein deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma. Recombinant human MCEE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Methylmalonyl CoA epimerase, DL methylmalonyl CoA racemase, EC 5.1.99.1, GLOD2, Glyoxalase domain containing 2. NCBI no.: NP_115990

Molecular Weight: 17.3 kDa (161aa) confirmed by MALDI-TOF

Pathways: Monocarboxylic Acid Catabolic Process