BPHL Protein (AA 38-291) (His tag)

Catalog No. ABIN667322

Product Details

Target: BPHL (Biphenyl Hydrolase-Like (Serine Hydrolase) (BPHL))

Protein Type: Recombinant

Protein Characteristics: AA 38-291

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This BPHL protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: BPHL, 38-291aa, Human, His tag, E.coli

Purity: > 95 % by SDS - PAGE

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl

Storage: 4 °C

Target Details

Target: BPHL (Biphenyl Hydrolase-Like (Serine Hydrolase) (BPHL))

Alternative Name: BPHL (BPHL Products)

Synonyms: 2010012D11Rik Protein, 5730533B08Rik Protein, AI115341 Protein, BPH-RP Protein, MCNAA Protein, VACVASE Protein, biphenyl hydrolase like Protein, biphenyl hydrolase-like (serine hydrolase, breast epithelial mucin-associated antigen) Protein, Bphl Protein, BPHL Protein

Background: BPHL is a Serine hydrolase which Belongs to the AB hydrolase superfamily. This family catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. This protein is expressed at high levels in liver and kidney and at lower levels in heart, intestine and skeletal muscle. It plays a role in detoxification processes. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. Recombinant human BPHL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Valacyclovir hydrolase, BPH-RP, MCNAA, VACVASE. NCBI no.: NP_004323

Molecular Weight: 31.1kDa (275aa), confirmed by MALDI-TOF