BCKDHA Protein (AA 46-445) (His tag)
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- Target See all BCKDHA Proteins
- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 46-445
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BCKDHA protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Characteristics
- BCKDHA, 46-445aa, Human, His tag, E.coli
- Purity
- > 80 % by SDS - PAGE
- Top Product
- Discover our top product BCKDHA Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl
- Storage
- 4 °C
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- Target
- BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))
- Alternative Name
- BCKDHA (BCKDHA Products)
- Synonyms
- wu:fd20d04 Protein, zgc:110049 Protein, BCKDE1A Protein, MSU Protein, MSUD1 Protein, OVD1A Protein, BCKDA Protein, E1a Protein, 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial Protein, branched chain keto acid dehydrogenase E1, alpha polypeptide Protein, 2-oxoisovalerate dehydrogenase alpha subunit (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain) (BCKDH E1-alpha) (BCKDE1A) Protein, branched chain ketoacid dehydrogenase E1, alpha polypeptide Protein, LOC704978 Protein, bckdha Protein, BCKDHA Protein, BckdhA Protein, Bckdha Protein
- Background
- BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: 2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSU, MSUD1, OVD1A. NCBI no.: NP_000700
- Molecular Weight
- 47.8kDa (421aa) confirmed by MALDI-TOF
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